Home > Sandwich ELISA Kits > Human Mas-related G-Protein Coupled Receptor Member D (MRGPRD) ELISA Kit

Product Name:

Human Mas-related G-Protein Coupled Receptor Member D (MRGPRD) ELISA Kit

Cat.#:

JL12268

Regulatory Status:

RUO

Alternative:

Gm499; Mrgd

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.156-10ng/mL

Sensitivity:

0.08ng/mL

Specificity:

This kit recognizes Human MRGPRD in samples. No significant cross-reactivity or interference between Human MRGPRD and analogues was observed.

Sample Type:

Serum;plasma;tissue homogenates;cell lysates;cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Cardiovascular Biology

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| MRGPRD

Mas-related G-Protein coupled receptor member D (MRGPRD) also referred to as hGPCR45 or TGR7, was identified as a novel GPCR in murine and human genomes. MRGD serves as the receptor of beta-alanine. Several MRG family members were expressed in specific subpopulations of sensory neurons, which detect pain stimuli. As for MRGD, its expression was found in dorsal root ganglia (DRG) and co-localized with Vanilloid receptor-1 (VR-1), which is an essential receptor for heat and pain sensation.MRGD is considered to be one of the players in pain sensation and/or transduction.MRGD transduces intracellular signaling of the angiotensin (Ang) II metabolite, Ang-(1–7).

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human MRGPRD spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	81-101	96
Plasma(n=8)	92-105	102
Cell Culture Supernates(n=8)	96-108	103

| Linearity

Samples were spiked with high concentrations of Human MRGPRD and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	86-96	90-110
1 : 2	Average (%)	91	93	96
1 : 4	Range (%)	89-103	87-108	105-115
1 : 4	Average (%)	92	98	108